Figure 3.

Functional assay to confirm suppression of induced silencing of the endogenous AeCPA-1 gene in midguts of PUbB2 P61 females. HWE and PUbB2 P61 females were intrathoracically injected with 1 µl of 1 µg/µl dsRNA targeting AeCPA-1 or with PBS (control). Twenty-four hours post-injection mosquitoes were given a bloodmeal. Following total RNA extraction from pools of 20 midguts at 7–48 h pbm, AeCPA-1 transcript copy number equivalents were quantified by qRT-PCR. NI = non-injected.