Fig. 3.

Effect of GW4064 treatment on liver and SI CYP3A expression in mice fed synthetic diet. Adult male WT mice fed the synthetic diet for 3 weeks were treated once with GW4064 by oral gavage or with the vehicle alone, and tissues were obtained 18 hours later for microsome preparation. Age-matched male WT mice fed with regular chow were also included for comparison. (A) Immunoblot analysis of CYP3A expression was performed as described in Fig. 1. Microsomes were prepared from pooled enterocytes of 2 mice in each group and analyzed in duplicates. Calnexin was analyzed as a loading control. Each lane contained 10 μg total protein. The results represent three independent experiments. (B) Results of densitometry analysis for relative CYP3A protein levels (mean ± S.D. [n = 3]; **P < 0.01; *P < 0.05, compared with the regular diet group.