Figure 4.

SDS-PAGE of centrifugal pellets (p) and supernatants (s) of Cecropia (A) and Monarch extracts (B). For Cecropia, P = pupa and A1,2 indicate days 1, and 2 after eclosion. For Monarchs, Af = adults freshly caught in the field; Ast = adults fed for four weeks under short day conditions; Alg = adults kept for two weeks in short days and then transferred to long days for an additional two weeks. The extract in each lane was a pool of the extracts from the indicated species and stage analyzed in Fig. 3. Asterisks indicate the 80 kDa level of the gels.