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. 2013 Feb;41(2):414–421. doi: 10.1124/dmd.112.047811

Fig. 3.

Fig. 3.

HPLC analysis of C-1305 and C-1311 metabolic transformation. (A and B) Nontransfected KB-3 cells or cells transiently transfected for 24 hours with UGT1A10 (KB-3/UGT1A10) were treated with 30 μM C-1305 (A) or 30 μM C-1311 (B) for 24, 48, or 72 hours, and methanol extracts were subjected to HPLC analysis as described under Materials and Methods. Top panels in (A) and (B): KB-3 cells; Bottom panels in (A) and (B): KB-3/UGT1A10 cells. Glucuronide products indicated by arrows. (C) KB-3 cells transiently transfected for 24 h with UGT2B4 (KB-3/UGT2B4) were treated with 30 μM C-1305 (left panel) or 30 μM C-1311 (right panel) for 24, 48, or 72 hours, and methanol extracts were subjected to HPLC analysis. Note the lack of glucuronide product.