Figure 7.

Polysaccharide analysis by carbohydrate gel electrophoresis of mannan polysaccharides in the cell wall of Arabidopsis primary stems. Cell walls [(alcohol-insoluble residue (AIR)] were digested using mannanases, and the resulting oligosaccharides were derivatized using 2-aminonaphthalene trisulfone followed by separation by polyacrylamide gel electrophoresis. Konjac glucomannan treated in the same way is shown for comparison. The asterisk indicates a non-specific band from a compound in the reaction reagents, and the bands quantified in Figure S3 are labeled ‘a’ and ‘b’. A representative gel is shown; the experiment was performed on three biological replicates, with two technical replicates of each. Man, mannose; Glc, Glucose.