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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: J Musculoskelet Neuronal Interact. 2012 Dec;12(4):209–218.

Fig. 1. Genotyping and confirmation of NPY overexpression Col2.3NPY mice.

Fig. 1

Col2.3NPY mice have been developed where NPY and GFP expression are driven by the 2.3-kb fragment of collagen type I promoter (A). GFP fluorescence of tail vertebrae (B) is used to distinguish Col2.3NPY (upper panels) and wild type (lower panels) mice. PCR for GFP confirmed Col2.3NPY genotype (C). Histology shows GFPtopaz expression in a subset of osteoblasts and osteocytes (indicated by arrowheads) in both females (D) and males (E). Expression of NPY mRNA in bone tissue of 2–3 month old mice was measured using qPCR (F), and NPY protein concentration in bone tissue (G) and serum (H) was quantified using EIA.