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. Author manuscript; available in PMC: 2013 Jan 30.
Published in final edited form as: Nat Biotechnol. 2012 May;30(5):460–465. doi: 10.1038/nbt.2170

Figure 1. Graphical overview of the FLASH assembly method.

Figure 1

(a) Archive of 376 TALE repeat encoding plasmids required to practice FLASH. Plasmids encoding one, two, three, and four TALE repeats (colored rectangles) harboring various RVDs (represented by two upper-case letters within the rectangles) were constructed as described in Methods. (b) Schematic overview of the FLASH assembly process. A DNA fragment encoding a single TALE repeat and labeled on its 5′ end with biotin (blue oval) is initially ligated to a second DNA fragment encoding four specific TALE repeats and then attached to a streptavidin-coated magnetic bead (orange sphere). Additional DNA fragments encoding pre-assembled TALE repeats are ligated until an array of the desired length is assembled. The DNA fragment encoding the full-length TALE repeat array is then cleaved from the bead by restriction digestion.