Figure 3. Computationally-derived design guidelines do not show statistically significant correlation with TALEN activity levels.

Each graph plots the mean EGFP-disruption activity of each of our 48 EGFP-targeted TALEN pairs against the number of half-sites within each target site that fail to meet one of four previously described design guidelines (Guidelines 2, 3, 4, or 5; see Supplementary Discussion¹⁷) or against the total number of guideline violations in each target site (Total Guideline Violations). Plots are shown for EGFP-disruption activities measured on day 2 and day 5 post-transfection. Guideline violation data are from Supplementary Table 3. Correlation p-values are shown for each plot.