TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source |
|---|---|---|
| E. coli | ||
| DH5α | Host for cloning vectors | Life Technologies |
| BL21 DE3/pLysE | Host for protein synthesis, Camr | Novagen |
| JMSB0338 | BL21 DE3/pLysE containing pJMSB0338; Ampr Camr | This work |
| S. enterica serovar Typhimurium | ||
| TR6579 | Shuttle vector between E. coli and S. enterica serovar Typhimurium LT2 | J. R. Roth |
| RMC26 | dxs::mevalonate operon (araC Pbad erg8 erg12 erg19 Kanr) | C. A. Testa |
| RMC29 | RMC26 with idi::Camr | This work |
| TT22236 | S. enterica serovar Typhimurium (LT2) cbiD24::MudJ containing pTP223 | J. R. Roth |
| JMSB0351 | RMC29 containing pJMSB0338 | This work |
| JMSB0354 | RMC29 containing pBAD Myc/HisA | This work |
| Plasmids | ||
| pGEM-T Easy | Cloning vector for PCR products; Ampr | Promega |
| pBAD Myc/His A | E. coli expression vector; Ampr | Invitrogen |
| pJMSB0335 | pGEM-T Easy with a 1.1-kb BgIII-HindIII fragment corresponding to bp 26230 through 27279 in the M. thermautotrophicus genome (ORF48) | This work |
| pJMSB0338 | pBAD Myc/His A with a 1.1-kb BgIII-HindIII fragment containing ORF48 | This work |
| pTP223 | lam, bet, and exo genes of phage lambda expressed from a lac promoter | A. R. Poteete and A. C. Fenton |
a
Camr, chloramphenicol resistance; Ampr, ampicillin resistance.