TABLE 6.

Promoter activities of sea-sed hybrid promoters^a

Plasmid	Sequenceb	CAT values (Agr wt/Agr Δ)c	ASId
sea mutant promoters
pZS2962 (wt)	TAGACAAATATAAAAAGTGTATAGTAATATATGTATGTATAATTAAATG	289.6 ± 7.1/305.6 ± 19.6	1.0
pZS2963	----------------------------ATGAAA---------------	365.9 ± 18.6/316.0 ± 17.1	1.2
pZS2964	ATGAAA-------------------------------------------	256.7 ± 23.2/164.8 ± 12.7	1.6*
pZS2965	ATGAAA----------------------ATGAAA---------------	66.9 ± 4.4/56.9 ± 6.7	1.2
pDT26	ATGAAA------------------TATAATGAAA---------------	33.6 ± 1.1/4.8 ± 0.5	6.8*
pDT27	------------------------TATAATGAAA---------------	295.4 ± 26.1/235.8 ± 19.9	1.3
sed mutant promoters
pZS2704 (wt)	ATGAAATGGATCAAATATATTGATATAATGAAAGTGAGCAAGTTGGATAGATTGCGGC	131.7 ± 6.1/58.2 ± 2.5	2.3*
pDT28	TAGACA---------------------------------------------	445.8 ± 24.8/333.4 ± 42.8	1.3*
pDT29	TAGACA-----------------TAATATATGT------------------	256.9 ± 14.4/213.2 ± 10.7	1.2*
pDT30	-----------------------TAATATATGT------------------	9.8 ± 0.7/3.9 ± 0.7	2.5*

^awt, wild type.

^bBoldface sequences are the −35 and −10 promoter elements. For hybrid promoters with the sea promoter as the backbone, the dashes represent bases identical to those in the wild-type sea sequence and the sed base substitutions are indicated. For hybrid promoters with the sed promoter as the backbone, the dashes represent bases identical to those in the wild-type sed sequence and the sea base substitutions are indicated.

^cMeans ± standard deviations (in nanomoles per milligram of cell dry weight per minute). Each value represents the average of at least four determinants. Agr Δ, agr mutant.

^dASI, Agr stimulation index (ratio of activity of the promoter in a wild-type host to that in an Agr deletion mutant). An asterisk indicates that results for agr mutant and agr⁺ hosts were significantly different.