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. Author manuscript; available in PMC: 2013 Mar 30.
Published in final edited form as: Circ Res. 2012 Mar 6;110(7):990–999. doi: 10.1161/CIRCRESAHA.112.264440

Figure 5.

Figure 5

Effect of R621E mutation on anion:cation permeability of mAno1. (A, B) Whole-cell recordings of (A) WT mAno1 in symmetrical 150 mmol/L NaCl with 180 nmol/L Cai and (B) R621E mAno1 in symmetrical 150 mmol/L NaCl with 1.1 μmol/L Cai. The R621E mutation decreased the Ca2+ sensitivity of the channel, requiring a larger Ca2+ concentration to generate a measurable current. (C, D) Current-voltage relationships of (C) WT mAno1 and (D) R621E mAno1 with different extracellular [NaCl]. (E) Change in reversal potential (ΔErev) for different extracellular [NaCl] or [CsCl] determined from experiments like those in C and D. Lines are best fits to the Goldman-Hodgkin-Katz equation.