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. 2013 Feb;19(2):289–292. doi: 10.3201/eid1902.120855

Table 2. Detection of IgM and IgG in serum samples from 2 men with human granulocytic anaplasmosis, Kochi Prefecture, Japan*.

Days after symptom onset Antibody titers, IgM/IgG†
R. japonica, cultured in L929 cells‡ O. tsutsugamushi, cultured in L929 cells§ Anaplasma phagocytophilum, propagated in
HL60 cells THP-1 cells
Case-patient 1
3 <20/<20 <20/<20 <20/<20 80/<20
19
<20/<20
<20/<20
<20/<20
320/80
Case-patient 2
4 <20/<20 <20/<20 20/<20 40/40
11 <20/320 <20/<20 <20/<20 160/80

*All Western blot testing using recombinant P44-1 antigen detected IgM and IgG; the antigen reacted with all sera tested, as shown in Figure 2. 
†Determined by using indirect immunofluorescence assay.
‡Rickettsia japonica strain YH.
§Orientia tsutsugamushi strains Gilliam, Karp, Kato, and Kawasaki.