Table 2. Detection of IgM and IgG in serum samples from 2 men with human granulocytic anaplasmosis, Kochi Prefecture, Japan*.
Days after symptom onset | Antibody titers, IgM/IgG† |
|||
---|---|---|---|---|
R. japonica, cultured in L929 cells‡ | O. tsutsugamushi, cultured in L929 cells§ |
Anaplasma phagocytophilum, propagated in |
||
HL60 cells | THP-1 cells | |||
Case-patient 1 | ||||
3 | <20/<20 | <20/<20 | <20/<20 | 80/<20 |
19 |
<20/<20 |
<20/<20 |
<20/<20 |
320/80 |
Case-patient 2 | ||||
4 | <20/<20 | <20/<20 | 20/<20 | 40/40 |
11 | <20/320 | <20/<20 | <20/<20 | 160/80 |
*All Western blot testing using recombinant P44-1 antigen detected IgM and IgG; the antigen reacted with all sera tested, as shown in Figure 2. †Determined by using indirect immunofluorescence assay. ‡Rickettsia japonica strain YH. §Orientia tsutsugamushi strains Gilliam, Karp, Kato, and Kawasaki.