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. 2013 Jan 28;19:135–145.

Table 1. List of primers for genes tested by qRT-PCR and annealing temperatures used for their respective target genes.

Genes Annealing temperature – band size
RPL27
55 °C - ~100 bp
Forward 5′ – 3′
ATTTATGAAACCCGGGAAGG
Reverse 5′ – 3′
CCAGGGCATGACTGTAAGGT
GAPDH
55 °C – 509 bp
Forward 5′ – 3′
GCCTCCTGTACTACCAACTG
Reverse 5′ – 3′
CCCCACTCGTTGTCATACCA
Cytochrome c oxidase subunit 2
61 °C – 355 bp
Forward 5′ – 3′
ACACTAACGCAATAGACGCACAAGA
Reverse 5′ – 3′
ACGCCCATTGAGGGGACTGC
Rad1
61 °C – 341 bp
Forward 5′ – 3′
TGCGTGCCTCGACAACGTCC
Reverse 5′ – 3′
TCACCACCCCACCTTCCTCCA
Stromelysin 1/2 a
61 °C – 168 bp
Forward 5′ – 3′
GGGGGACAAAGACTCTCCCCGA
Reverse 5′ – 3′
CTGGTGTTCTTCAGTGTCCGGGT
VEGFR1
61 °C – 227 bp
Forward 5′ – 3′
TCCTGCAGCAGCCTGACCTTG
Reverse 5′ – 3′
GTTTGGGGCTGTGACTCGGC
Avidin
60 °C – 103 bp
Forward 5′ – 3′
TCGTTTCTCCTCTGACGGGCT
Reverse 5′ – 3′
TGCCCTGCCCAGGTGGTGAT
Glutathione peroxide 1
60 °C – 104 bp
Forward 5′ – 3′
GCTGGTGGTGCTGGGCTTCC
Reverse 5′ – 3′ ACCCTTTTCCTGGACGAACGTACT