Table 1. List of primers for genes tested by qRT-PCR and annealing temperatures used for their respective target genes.
Genes | Annealing temperature – band size |
---|---|
RPL27 |
55 °C - ~100 bp |
Forward 5′ – 3′ |
ATTTATGAAACCCGGGAAGG |
Reverse 5′ – 3′ |
CCAGGGCATGACTGTAAGGT |
GAPDH |
55 °C – 509 bp |
Forward 5′ – 3′ |
GCCTCCTGTACTACCAACTG |
Reverse 5′ – 3′ |
CCCCACTCGTTGTCATACCA |
Cytochrome c oxidase subunit 2 |
61 °C – 355 bp |
Forward 5′ – 3′ |
ACACTAACGCAATAGACGCACAAGA |
Reverse 5′ – 3′ |
ACGCCCATTGAGGGGACTGC |
Rad1 |
61 °C – 341 bp |
Forward 5′ – 3′ |
TGCGTGCCTCGACAACGTCC |
Reverse 5′ – 3′ |
TCACCACCCCACCTTCCTCCA |
Stromelysin 1/2 a |
61 °C – 168 bp |
Forward 5′ – 3′ |
GGGGGACAAAGACTCTCCCCGA |
Reverse 5′ – 3′ |
CTGGTGTTCTTCAGTGTCCGGGT |
VEGFR1 |
61 °C – 227 bp |
Forward 5′ – 3′ |
TCCTGCAGCAGCCTGACCTTG |
Reverse 5′ – 3′ |
GTTTGGGGCTGTGACTCGGC |
Avidin |
60 °C – 103 bp |
Forward 5′ – 3′ |
TCGTTTCTCCTCTGACGGGCT |
Reverse 5′ – 3′ |
TGCCCTGCCCAGGTGGTGAT |
Glutathione peroxide 1 |
60 °C – 104 bp |
Forward 5′ – 3′ |
GCTGGTGGTGCTGGGCTTCC |
Reverse 5′ – 3′ | ACCCTTTTCCTGGACGAACGTACT |