Table 1. Primers for quantitative PCR.
Genes | Primers (5′-3′) |
---|---|
PAC1 |
F: GTGGCTGTTCTCTACTGTTTTCTGA |
R: GCTTCGCCATTTTCGCTTGATC |
|
Probe: TCCGCTTGTACCTCGCC |
|
VPAC1 |
F: CCCCTCATCTTCAAGCTCTTCTC |
R: CAGGTGTGTCCAACCTTCGT |
|
Probe: CACATTGCGGCCTTGAAT |
|
VPAC2 |
F: CTCTGATGTCTCTTGCAACAGGAA |
R: CAGGAACAGGTTCAGATGGATGTAA |
|
Probe: CCTCTTCAGGAAGCTGC |
|
GAPDH |
F: TGCACCACCAACTGCTTA |
R: CATGAGTCCTTCCACGATACCAA |
|
Probe: CCCTGGCCAAGGTCATCCATGA |
|
HPRT1 |
F: TCCATTCCTATGACTGTAGATTTTATCAGACT |
R: AGTTGAGAGATCATCTCCTCCGATT |
|
Probe: CCTGTTGACTGGTCATTACA |