Table 2.

Minimum inhibitory concentrations (MIC) of the CH₂Cl₂: MeOH (1:1) extract, fractions and compounds of Brillantaisia lamium

Test substances	Minimum inhibitory concentrations (in µg/ml)
	Bacteria				Fungi
	Enterococcus faecalis	Staphylococcus aureus	Salmonella typhi	Proteus mirabilis	Candida albicans	Candida tropicalis	Cryptococcus neoformans
Crude extract	125	250	125	62.50	500	250	250
Fraction A	na	na	na	na	na	na	na
Fraction B	1000	500	na	na	na	500	na
Fraction C	250	500	na	na	1000	500	1000
Fraction D	125	125	62.50	62.50	125	62.50	125
Fraction E	na	500	na	na	1000	500	na
Mixture of sterols	50	100	na	200	na	400	na
1	100	50	50	100	50	100	12.50
2	400	200	na	200	200	na	200
3	25	6.25	12.50	12.50	50	50	6.25
4	100	100	na	100	200	100	50
Gentamicin	12.50	50	50	100	nt	nt	nt
Nystatin	nt	nt	nt	nt	1.56	6.25	1.56

Tabulated values are the means of three trials which did not show any variation (SD=0); na: not active at concentrations up to 400 µg/ml (for pure compounds and mixture of sterols) or 1 mg/ml (for crude extract and fractions); nt: not tested; gentamycin and nystatin are used as reference antibiotics for bacteria and yeasts respectively; aurantiamide acetate (1); lupeol (2); lespedin (3); sitosterol 3-O-β-D-glucopyranoside (4); a mixture of sterols: campesterol (5); stigmasterol (6); β-sitosterol (7).