Figure 7A.

Western blot of the 40 kDa mosquito protein using a commercially available rabbit polyclonal c-JUN primary antibody (epitope corresponding to the highly conserved DNA binding domain of mouse) from Santa Cruz Biotechnology Inc. (Santa Cruz, CA) known to be mouse, rat, chicken and human reactive. Lane 1 is the negative control without the primary antibody. Lane 2 shows ∼ 2 µg of purified protein, and lane 3 contains 25 µg of nuclear protein lysate. Primary antibody was used at a dilution of 1: 1000. The secondary antibody (goat anti-rabbit IgG tagged with alkaline phosphatase) was obtained along with the development kit from Biorad Laboratories (Hercules, CA).