Fig. 6.

a: endo-Glycosylceramidase hydrolyzes glycosphingolipids to produce a glycan and ceramide. Lactosyl ceramide (LacCer) is used as a substrate in this experiment. b: Results of the analysis of the anomers of lactose produced by the endo-glycosylceramidase. The estimated plateau values obtained from the ERMS spectra for ions with m/z 203, 305 and 347 are presented. The reaction was terminated after 15 min (blue open circle) and 60 min (blue closed circle). Red closed circle: equilibrated lactose (in the reaction medium); “+”: estimated value for pure β-anomer (The data presented in Fig. 2c were recalculated using the common ions.); “×”: equilibrated lactose (in the water); Error bar: 95% confidence interval.