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Response surfaces for optimization step 1. (A) ACAN:VCAN expression ratio as a response to the factors TGF-β1 and glucose. (B) COLII:COLI expression ratio as a response to the factors TGF-β1 and glucose. (C) SOX9 gene expression as a response to the factors TGF-β1 and dexamethasone. ACAN, aggrecan; VCAN, versican; COL, collagen; SOX9, sex determining region Y-box 9 protein.
