FIG. 4.

Potential of proliferated ASCs (A) and BMSCs (B) in the low-concentration HP–DMEM gel with FGF-2 to form adipogenic phenotype cells. Top image of each panel: ASCs and BMSCs proliferated in high-concentration HS (10%)–DMEM without FGF-2 were cultured in adipogenic induction (maintenance) medium (positive [P] control) or control medium (negative [N] control) for 21 days. Both 3D-proliferated cell types in the low-concentration HP–DMEM gel with F/P NPs and FGF-2 were cultured with either adipogenic induction (maintenance) medium on F/P NP–coated (+) or uncoated tissue culture plates (−) for 21 days. Cells were stained with Oil Red O. Lower images of each panel: Magnifications of negative-control and F/P NP–coated plate cultures. Scale bars=100 μm.