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Comparison of the Efficiency of Human Embryonic Stem Cell Derivation Using Blastocysts Developed from Frozen–Thawed and Fresh Embryos
| Embryos | Blastocysts used n | Attached n (%) | ICM outgrowth n (%) | hES-like cells n (%) | Cell lines n (%) | Name |
|---|---|---|---|---|---|---|
| Frozen–thawed | 5 | 5 (100.0) | 3 (60.0) | 1 (20.0) | 1 (20.0) | Chula2.hES |
| Fresh | 8 | 7 (87.5) | 5 (62.5) | 3 (37.5) | 2 (25.0) | Chula4.hES Chula5.hES |
| Total | 13 | 12 (92.3) | 8 (61.5) | 4 (30.8) | 3 (23.1) |
ICM, inner cell mass; hES, human embryonic stem.
