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. 2012 Dec;1(6):273–279. doi: 10.1089/biores.2012.0252

FIG. 2.

FIG. 2.

TERT expression in hMSCs and hMSC TERT cells. (A) Relative changes in TERT mRNA expression were determined by real-time reverse transcription (RT)–polymerase chain reaction (PCR) and expressed in relation to 18S levels, and the reference point [mock-transduced hMSC P4(8) levels] using the 2−ΔΔC(T) method. hMSCs have a low, but detectable, level of TERT mRNA expression at P4(8), but with continued passage TERT expression quickly decreases to nearly undetectable levels even by early-passage P6(10). hMSC TERT cells have significantly high levels of TERT mRNA at all passages, even late passage P16(20). *p<0.05 compared to hMSC levels at each passage. (B) TERT enzymatic activity was also measure at both early [P6(10)] and late [P16(20) and P26(30)] passages. There are significantly higher levels of TERT activity in hMSC TERT cell extracts than in hMSC extracts throughout the time course of the study. *p<0.05 compared to hMSC levels at P6(10).