FIG. 6.

Differentiation of hMSC TERT cells toward the osteoblastic and adipogenic lineages. hMSC TERT cells were treated with an osteogenic medium (OGM) for 14 or 28 days. (A) Relative changes in alkaline phosphatase (ALP) expression were determined by real-time RT-PCR. ALP mRNA expression is significantly upregulated at 7, 10, and 14 days. *p<0.05 compared to hMSC TERT levels at day 0. (B) Alizarin Red staining of early- [P5(9)] and late- [P16(20)] passage cells demonstrates that the hMSC TERT cells can undergo terminal differentiation, resulting in the deposition of mineral after OGM treatment for 28 days. (C) Oil Red-O staining of lipid droplets confirms that the hMSC TERT cells can also undergo adipogenic differentiation after induction for 21 days.