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MSC were induced to chondrogenic lineage cells. Their chondrogenic ability was assessed via (A) alcian blue and (C) collagen type II staining, while the controls were negative for both (B) alcian blue and (D) collagen type II staining. (E) After 28 days of induction, the chondrogenic MSC showed significant expression of cartilage-specific genes SOX9 and COL2A1 as compared to negative controls (NC) and undifferentiated MSC, day 0. Bar: 500 μm.
