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On morphological evaluation, ECM showed two prominent forms, (A) particle-like appearance and (B) small membrane-like structures (inset is a higher resolution image, bar: 20 μm). The particle-like structures of ECM were (C) positive for alcian blue and (E) marginally positive for collagen type II staining. The membrane-like structures of ECM were equally positive for both (D) alcian blue and (F) collagen type II staining. Insets (D, F): ECM from control samples. Bar: 100 μm.
