FIG. 5.

Reovirus enhances NK-mediated cytotoxicity against AML cells. Whole PBMC were isolated from blood of healthy donors, cultured overnight with 0 or 0.1 pfu/cell reovirus, and then assessed as follows: (a) PBMC were incubated with ⁵¹Cr-labeled AML cell lines for 4 h at different effector:target ratios. Percentage target cell lysis was then determined. Results show mean±s.e.m. of four independent experiments. (b) Whole PBMC, CD56-depleted PBMC, or PBMC treated with 2 mM EGTA were incubated with ⁵¹Cr-labeled Kasumi-1 or THP-1 cell lines for 4 h, at different effector:target ratios. Percentage target cell lysis was then determined. Results are representative of four independent experiments. EGTA, ethylene glycol tetraacetic acid.