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. 2012 Jan;1(1):3–15. doi: 10.1089/biores.2012.0205

FIG. 6.

FIG. 6.

Reovirus enhances the activity of NK cells toward AML in patient samples. (a) PBMC from an AML patient were incubated overnight in the presence of absence of 0.1 pfu/cell reovirus. Cells were then incubated with THP-1 cells (at a 10:1 ratio) for 5 h. Cell-surface CD107a/b expression within the NK cell population was determined using flow cytometry. (b) Healthy donor PBMC were incubated overnight in the presence or absence of 0.1 pfu/cell reovirus. PBMC were then incubated with primary AML blasts (at a 10:1 ratio) for 5 h. Cell-surface CD107a/b expression within the NK cell population was determined using flow cytometry. Each donor is represented by a separate line. p<0.05 for targets 1 and 2; p=0.069 for target 3. (c) Healthy donor PBMC were incubated overnight in the presence or absence of 0.1 pfu/cell reovirus. PBMC were then incubated with primary AML blasts (at a 10:1 ratio) for 5 h. Intracellular IFNγ production within the NK cell population was determined using flow cytometry. Graph shows mean+s.e.m. of four individual donors. p=0.058 for target 1; p<0.05 for target 2; p=0.184 for target 3).