FIG. 4.

Scaffold-free neocartilage. (A) Image of articular neocartilage after 8 weeks of culture. (B) Image of auricular neocartilage after 8 weeks of culture. (C–D) Safranin O-stained histological micrographs of (A, B), respectively. Safranin O staining corresponds to glycosaminoglycan (GAG) content. White arrowheads show the surface, which was adjacent to the membrane during the culture, and black arrowheads show the free surface. (E) Opticell^™ chamber and developing neocartilage. An edge of the neocartilage is shown by a white arrowhead. (F) Safranin O-stained cross section of auricular neocartilage produced in an Opticell chamber.