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Amplification of prions from ARQ/ARQ scrapie-affected flock/farm mates of scrapie-infected sheep, 297/97. Unamplified samples (lanes 1, 3 and 6) or amplified for 4 rounds in ARQ/ARQ substrate (lanes 2, 4 and 8) or VRQ/VRQ substrate (lane 7). Molecular mass markers are shown (M, kDa). Unseeded ARQ/ARQ substrate subjected to PMCA amplification is shown in lane 5. In all lanes 0.3 mg wet tissue equivalent loaded per lane).
