Table 2.
PMCA-amplification of infected flock/farm mates of ARQ/ARQ scrapie-infected sheep
| Farm* | Sheep ID | Breed | Age at death |
PMCA substrates# |
|
|---|---|---|---|---|---|
| VRQ/VRQ | ARQ/ARQ | ||||
| 12 |
913/05 |
Mule |
5y 0 m |
+ |
+ |
| SS0-560272 |
Suffolk Cross |
4y |
+ |
+ |
|
| 15 |
1230/04 |
Suffolk Cross |
2y 4 m |
+ |
+ |
| 709/02 |
Cheviot/Shetland |
5y |
+ |
+ |
|
| 768/02 |
Cheviot/Shetland |
4y |
+ |
+ |
|
| 857/04 |
Cheviot/Shetland |
7y 3 m |
+ |
+ |
|
| 858/04 |
Cheviot/Shetland |
3y 3 m |
+ |
+ |
|
| 872/04 |
Cheviot/Shetland |
7y 3 m |
+ |
+ |
|
| 13 |
960/06 |
White Face Dartmoor |
7y |
+ |
+ |
| 703/07 |
White Face Dartmoor |
6y |
+ |
+ |
|
| 912/07 |
White Face Dartmoor |
7y |
+ |
+ |
|
| 11 | 297/97 |
Finn Dorset |
not known |
- |
+ |
| 210/03 |
Warborough |
2y 2 m |
+ |
+ |
|
| 209/03 |
Warborough |
5y 1 m |
- |
+ |
|
| 1583/97 |
Finn Dorset |
4y |
- |
+ |
|
| 1685/97 |
Finn Dorset |
4y |
- |
+ |
|
| 1002/03 |
Finn Dorset Cross |
7y |
- |
+ |
|
| 1158/00 |
Finn Dorset |
6y |
- |
+ |
|
| 57/99 | Finn Dorset | 5y 1 m | - | + | |
*Farms 12, 15, 13 and 11 were located in Gloucestershire, Aberdeenshire, Devon and Oxfordshire respectively. Samples were analysed after 4 rounds of PMCA.
#Each sample was amplified twice using a single substrate of each genotype and a positive sample was recorded when positive in at least one replicate.