Table 4.
Amplification of experimental and natural strains of ovine prion diseases
| Strain | Genotype |
Substrate |
||
|---|---|---|---|---|
| VRQ | ARQ | AFRQ* | ||
| SSBP1 (n = 1) |
VRQ |
+ |
- |
ND |
| Dawson (n = 1) |
VRQ |
+ |
- |
ND |
| MRI Suffolk (n = 3) |
ARQ |
−/−/− |
+/−/− |
ND |
| Atypical scrapie (n = 7) | ARQ (n = 5) or ARR (n = 2) | −/−/−/−/−/− | −/−/−/−/−/− | −/−/−/−/−/− |
n = number of animals tested. Samples were tested in duplicate in separate assays *AFRQ/AFRQ substrate where F represents amino acid at position 141. The AFRQ/AFRQ genotype is more commonly associated with atypical scrapie [19]. ND = not done. +/− symbols show the amplification outcome for each animal.