Table 1.
Primer Design for Quantitative RT-PCR Analysis
| mRNA | Primersa | Annealing Tm (cycle) |
|---|---|---|
| Aggrecan |
Fw: 5′-TGAGGAGGGCTGGAACAAGTACC-3′ |
58°C (32) |
| Rv: 5′-GGAGGTGGTAATTGCAGG GAACA-3′ | ||
| Col II |
Fw: 5′-AACACTGCCAACGTCCAGAT-3’ |
58°C (32) |
| Rv: 5′-CTGACGCACGGTATAGGTGA-3’ | ||
| ADAMTS-4 |
Fw: 5’-GTCTGTGTC CAGGGCCGATGC-3’ |
55°C (32) |
| Rv: 5’-GCCGCCGAAGGATCTCCAGAA-3’ | ||
| ADAMTS-5 |
Fw: 5’-GCGGATGTGTGCAAGCTGACC-3’ |
55°C (32) |
| Rv: 5’-AGTAGCC CATGCCATGCAGGA-3’ | ||
| MMP-1 |
Fw: 5’-TCAGTTCGTCCTCACTCCAG-3’ |
58°C (32) |
| Rv: 5’-TTGGTCCACCTGTCATCTTC-3’ | ||
| MMP-3 |
Fw: 5’-ATGGACCTTCTTCAGCAA-3’ |
58°C (32) |
| Rv: 5’-TCATTATGTCAGCCTCTC-3’ | ||
| MMP-13 |
Fw: 5’-AGGAGCATGGCGACTTCTAC-3’ |
58°C (32) |
| Rv: 5’-TAA AAA CAG CTC CGC ATC AA-3’ | ||
| TIMP-1 |
Fw: 5’-GCA ACTCCGACCTTGTCATC-3’ |
58°C (32) |
| Rv: 5’-AGCGTAGGTCTTGGTGAAGC-3’ | ||
| TIMP-3 |
Fw:5’-GGCGGCAGCAGCGGCAATGAC-3’ |
58°C (32) |
| Rv:5’-TACCAGCTTCTTCCCCACCACCTT-3’ | ||
| β-actin |
Fw: 5’-GCTCTCCAGAACATCACTCCTGCC-3’ |
58°C (27) |
| Rv: 5’-CGTTGTCATACCAGGAAATGAGCTT-3’ | ||
aFw, forward; Rv, reverse; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Tm, temperature