Skip to main content
. 2013 Jan 30;8(1):e55147. doi: 10.1371/journal.pone.0055147

Figure 1. The molecular characterization of the t(14;17)(q32;q22-23) translocation in ATLL-31.

Figure 1

(A) A schematic representation of the BCL11B locus on chromosome 14q32 (upper), the VMP1 locus on chromosome 17 (middle) and the t(14;17) locus (bottom). The arrowheads indicate the primer positions used to confirm the rearrangement by PCR. (B) The Southern hybridization of EcoRI-digested genomic DNA from PBMCs of ATLL-31 using probe A and normal placental DNA as a control. The vertical lines denote EcoRI sites. (C) The nucleotide sequence analysis of the breakpoint junction exhibited in the ATLL-31 case and the alignment of the corresponding germline regions. The germline sequences of chromosomes 14 and 17 are indicated with capital and lowercase letters, respectively. The vertical lines show sequence identity. The common nucleotides at the breakpoint junction are boxed. (D) The genomic DNA from PBMCs of the ATLL-31 case and a normal volunteer was subjected to genomic PCR analysis using primers A and B to amplify the breakpoint region.