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. 2013 Jan 30;8(1):e55338. doi: 10.1371/journal.pone.0055338

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© 2013 Kim et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Real-time RT-PCR was carried out to measure the pre-mRNA (A) and mature-mRNA levels (B) of the three selected genes. Primers spanning an exon and its downstream intron were used for pre-mRNA and primers spanning two exons were used for mature-mRNA. Gray and black bars denote the expression of the indicated genes for non-treated and treated with tetracycline in the ΔLF cells, respectively. Each clone was analyzed in duplicate, and the average relative expression level of 15 clones is presented with the standard error. *Significant (P<0.05; Student's t-test).