Figure 3. Calbindin and VGLUT1 staining in cerebellum of early stage 22L-animals highlights dominant dendritic pathology in early disease.

Co-labelling of Purkinje cells using the anti-calbindin antibody (green) and parallel fibers (VGLUT1 antibody, red) at increasing magnifications from panel A to C in 22L-animals at ES p.i. The VGLUT1 immunoreactivity remains preserved in the areas of dendritic disintegration as revealed by discontinuous outlines of Purkinje dendrites (B, C). There is no significant loss of neurons at this time (nuclear stain, blue). Scale bars: 405 µm (A); 88 µm (B); 61 µm (C).