Skip to main content
. 2013 Jan 30;8(1):e55186. doi: 10.1371/journal.pone.0055186

Figure 1. Identification of p-Smad2/3 inducible miRNAs.

Figure 1

(A) Western blot analysis of p-Smad2 and PCNA (loading control) levels in TAG1 ES cells untreated (0), treated with SB-431542 (SB) for 16 hrs (16) or with SB for 16 hrs followed by doxycycline (Dox) for 12 hrs (16+12). (B) Relative fold induction of mature miRNAs in TAG1 cells treated as in (A) detected by small RNA deep-sequencing. (C) qPCR validation of the deep-sequencing data, normalized to the non-Smad2/3 responsive miR-26a. Error bars represent ±SEM of triplicate reactions.