Table 1. Identification of Smad2/3 inducible miRNAs by small RNA deep-sequencing and real-time qPCR.
miRNA | TAG1 | J1 | |||||||||
Total reads | Normalized reads | Fold-change | |||||||||
0 h | 16 h | 16+12 h | 0 h | 16 h | 16+12 h | 16h v 0h | 16+12h v 16h | ||||
DS | qPCR | DS | qPCR | ||||||||
485-3p | 15123 | 3204 | 14883 | 1380 | 282 | 1504 | −4.89 | 5.33 | |||
181d-5p | 107042 | 42731 | 183584 | 9770 | 3763 | 18552 | −2.60 | −2.07 | 4.93 | 4.36 | 1.09 |
423-5p | 62261 | 30061 | 112927 | 5683 | 2647 | 11412 | −2.15 | 1.12 | 4.31 | 1.11 | 1.32 |
25-5p | 15513 | 8015 | 29645 | 1416 | 706 | 2996 | −2.01 | 4.24 | |||
320-3p | 90928 | 52727 | 174984 | 8300 | 4644 | 17683 | −1.79 | 1.04 | 3.81 | 1.16 | 1.2 |
7a-5p | 12989 | 9111 | 28023 | 1186 | 802 | 2832 | −1.48 | −2.58 | 3.53 | 2.8 | 1.07 |
128-3p | 10758 | 6212 | 14348 | 982 | 547 | 1450 | −1.79 | 2.65 | |||
181c-5p | 11420 | 6098 | 13507 | 1042 | 537 | 1365 | −1.94 | −1.76 | 2.54 | 3.66 | −1.01 |
295-5p | 15726 | 10758 | 22723 | 1435 | 947 | 2296 | −1.52 | 2.42 | |||
543-3p | 77103 | 24436 | 50833 | 7038 | 2152 | 5137 | −3.27 | 2.39 | |||
298-5p | 20568 | 13599 | 27069 | 1877 | 1198 | 2735 | −1.57 | 2.28 | |||
323-3p | 12677 | 6410 | 12510 | 1157 | 565 | 1264 | −2.05 | 2.24 | |||
290-5p | 390434 | 205450 | 370150 | 35638 | 18094 | 37406 | −1.97 | −1.3 | 2.07 | 1.46 | −1.04 |
485-5p | 11901 | 5602 | 9914 | 1086 | 493 | 1002 | −2.20 | −1.42 | 2.03 | 1.1 | 1.09 |
433-3p | 16712 | 6807 | 11648 | 1525 | 599 | 1177 | −2.54 | 1.96 | |||
744-5p | 16396 | 12174 | 20521 | 1497 | 1072 | 2074 | −1.40 | −1.54 | 1.93 | 1.59 | −1.09 |
382-5p | 21060 | 11862 | 19882 | 1922 | 1045 | 2009 | −1.84 | −1.69 | 1.92 | 1.45 | 1 |
672-5p | 43113 | 19036 | 31043 | 3935 | 1676 | 3137 | −2.35 | 1.87 | |||
669c-5p | 15701 | 12393 | 19642 | 1433 | 1091 | 1985 | −1.31 | 1.82 | |||
185-5p | 26062 | 17301 | 27039 | 2379 | 1524 | 2732 | −1.56 | −1.34 | 1.79 | 1.28 | 1.09 |
541-5p | 28812 | 19793 | 30572 | 2630 | 1743 | 3089 | −1.51 | 1.77 | |||
92a-3p | 58724 | 36877 | 55151 | 5360 | 3248 | 5573 | −1.65 | 1.72 | |||
130a-3p | 15364 | 11642 | 16887 | 1402 | 1025 | 1707 | −1.37 | −1.32 | 1.66 | 1.65 | 1.02 |
363-5p | 24461 | 13906 | 19919 | 2233 | 1225 | 2013 | −1.82 | 1.64 | |||
291a-5p | 28030 | 21958 | 29674 | 2558 | 1934 | 2999 | −1.32 | 1.55 | |||
25-3p | 140851 | 111946 | 148185 | 12856 | 9859 | 14975 | −1.30 | −1.61 | 1.52 | 1.71 | 1.05 |
341-3p | 14130 | 9000 | 11191 | 1290 | 793 | 1131 | −1.63 | 1.43 | |||
382-3p | 12866 | 7865 | 9310 | 1174 | 693 | 941 | −1.70 | 1.36 |
DS = Deep-sequencing.