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. 2013 Jan 30;8(1):e55155. doi: 10.1371/journal.pone.0055155

Figure 2. OASIS promotes the transcription of VEGFA.

Figure 2

(A) Schematic diagram of the human VEGFA promoter region. The first intron of the human VEGFA gene contains an ATF4-binding site (○), and that the 6-kbp 5′-upstream promoter has two potential binding sites for XBP1 (•) and CRE-like sites (▪). TSS: transcription start site. (B) Reporter assays using ARPE-19 cells. A reporter vector derived from the 6-kbp 5′-upstream region of the human VEGFA gene and expression vectors for XBP1, ATF4, ATF6, or OASIS were co-transfected. At 48 h after the transfection, luciferase activities were measured. Data are means ± SD (n = 4). *p<0.05, ***p<0.001, by Student’s t-test. (C) RT-PCR analysis of VEGFA in ARPE-19 cells infected with an adenovirus vector carrying OASIS. The right panel shows the results of real-time RT-PCR. The VEGFA mRNA expression level is increased by 5.5-fold after the transfection of OASIS. Data are means ± SD (n = 3). **p<0.01, by Student’s t-test.