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. 2013 Jan 30;8(1):e55155. doi: 10.1371/journal.pone.0055155

Figure 4. OASIS acts on the CRE-like site at –509 to –506 bp in the VEGFA promoter.

Figure 4

(A) The top panel shows schematic diagrams of the mutated reporter constructs. The bottom panel shows schematic representations of the wild-type CRE-like site (containing an ACGT core) and the mutated CRE-like sites (containing an AaGg core). (B) Reporter assays using ARPE-19 cells. Each mutated reporter vector and the OASIS N-terminus expression vector were co-transfected. Reporter assays were performed at 48 h after the transfection. Note that reporter activities significantly decreased in cells transfected with the mutated CRE-like site 4 construct. Data are means ± SD (n = 4). ***p<0.001, by Student’s t-test. (C) Western blot analysis shows the FLAG-tagged OASIS N-terminus was expressed at equal levels in each sample.