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. 2013 Jan 30;8(1):e55155. doi: 10.1371/journal.pone.0055155

Figure 5. OASIS directly binds to the promoter region in the human VEGFA gene.

Figure 5

(A) Schematic representation of the VEGFA promoter and the annealing sites of the primer set used in the ChIP assays. (B) PCR amplification of the VEGFA promoter region including the CRE-like site 4. ARPE-19 cells were transfected with a vector expressing the FLAG-tagged OASIS N-terminus. A GFP expression vector was used as a control. Immunoprecipitation was performed with anti-histone H3, anti-mouse IgG, or anti-FLAG antibodies, followed by the PCR using the specific primer sets.