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. 2013 Jan 30;8(1):e55665. doi: 10.1371/journal.pone.0055665

Figure 1. TMZ induces autophagy in LN-229 cells.

Figure 1

(a) MDC staining of autophagic vacuoles 96 h after TMZ treatment (100 µM)and (b) LC3-GFP vacuoles in LN-229 cells after TMZ treatment (100 µM), TO-PRO-3 was used for nuclear staining. (c) Quantification of MDC positive vacuoles 24–144 h after TMZ treatment in LN-229 cells. (d) Quantification of LC3-GFP positive vacuoles 96 h after TMZ treatment in LN-229 cells. (e) LC3B expression 96 h after TMZ treatment (100 µM) performed by western blot, ERK-2 was used as loading control. (f) Quantification of MDC positive vacuoles 24–144 h after TMZ treatment (100 µM) in U87 MG cells.