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. 2013 Jan 30;8(1):e55665. doi: 10.1371/journal.pone.0055665

Figure 4. TMZ-induced autophagy comes earlier than apoptosis and inhibits it.

Figure 4

Autophagy and apoptosis as determined by MDC staining and Sub-G1 flow cytometry 48–144 h after TMZ treatment (100 µM) in (a) LN-229 and (b) U87 MG cells. Apoptosis and necrosis was determined by Annexin V-FITC/PI double staining and quantified by flow cytometry 120 h after TMZ treatment in the absence or presence of 3-methyladenine (3-MA, 5 mM, 24 h after TMZ treatment) in (c) LN-229 and (d) U87 MG cells. (e) Autophagy determined by MDC staining and quantified via flow cytometry 96 h after TMZ treatment (100 µM) in LN-229 cells in the absence or presence of 3-MA (5 mM, 24 h after TMZ treatment). (f) Apoptosis and necrosis determined by Annexin V-FITC/PI double staining and quantified by flow cytometry 96 h after TMZ treatment in the absence or presence of 3-methyladenine (3-MA, 5 mM, 24 h after TMZ treatment) in LN-229 cells.