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. 2013 Jan 30;8(1):e55665. doi: 10.1371/journal.pone.0055665

Figure 5. TMZ induces autophagy through activation of ATM.

Figure 5

(a) Western blot analysis of p-ATM (Ser-1981), ATM and LC3B in LN-229 cells 48–96 h after TMZ treatment (20 µM and 100 µM correspondingly) Tallin-1 and ERK-2 were used for loading control. (b) Autophagy positive cells as determined by MDC staining 96 h after TMZ treatment and (c) apoptosis and necrosis as determined by Annexin V-FITC/PI double staining 120 h after treatment and quantified by flow cytometry in LN-229 cells transiently knocked-down for ATM (or transfected with scrambled siRNA) treated with TMZ (100 µM). (d) Western blot analysis of ATM protein levels of LN-229 cells transiently knocked-down for ATM (or transfected with scrambled siRNA) 24–96 h after transfection. Tallin-1 was used as loading control.