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. 2013 Jan 30;8(1):e55646. doi: 10.1371/journal.pone.0055646

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© 2013 Sabirov et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Dependence of the GSH concentration in the extracellular fluid on the number of cells in suspension under control isotonic (290 mosmol/kg-H₂O) conditions (filled symbols) and upon hypotonic (147 mosmol/kg-H₂O) stimulation (open symbols). The cells were incubated 10 min at 25^oC. (B) Time course of GSH release from rat thymocytes under control isotonic conditions (filled symbols) and upon hypotonic (147 mosmol/kg-H₂O) stimulation (open symbols). The suspension containing 1 x 10⁸ cells/ml was incubated at 25^oC for the indicated time. (C) Osmolality dependence of GSH release from rat thymocytes. The suspension containing 1 x 10⁸ cells/ml was incubated at 25^oC for 20 min in solutions with various tonicity. *Significantly different from the isotonic control values at P<0.05.