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. 2013 Jan 30;8(1):e55646. doi: 10.1371/journal.pone.0055646

Figure 4. Effects of organic anion transporter substrates and inhibitors on the swelling-induced GSH release from rat thymocytes.

Figure 4

Stimulating effects of the SLCO/OATP substrates, probenecid (A), taurocholic acid (B) and estrone sulfate (C), and a suppressing effect of the inhibitor of SLC23A/OAT transporter, p-aminohippuric acid (PAH), alone and in combination with VSOR blockers, phloretin (200 µM) (D) and DCPIB (20 µM) (F) on the net GSH release from rat thymocytes upon hypotonic stimulation are shown. The suspension containing 1×108 cells/ml was incubated for 20 min at 25 °C in the hypoosmotic (147 mosmol/kg-H2O) conditions in the absence of any drug (control) or presence of the above-indicated drugs used at the concentration of 500 µM (A), 2 mM (B and C) and 1 mM (D and F). In order to minimize day-to-day variations, the results shown in (D) and (F) were obtained within one experiment performed in one day. *Significantly different from the control values at P<0.05. #Significantly different from phloretin (D) or DCPIB (F) alone at P<0.05.