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. 2013 Jan 30;8(1):e55646. doi: 10.1371/journal.pone.0055646

Figure 6. Glutamate and GSH permeability of the whole-cell currents activated by cell swelling in rat thymocytes.

Figure 6

(A) Whole-cell current responses to ramp pulses from −70 to +70 mV before and after replacement of extracellular Cl− with glutamate. The pipette solution was Cl−-rich (125 mM CsCl); the bath solution was normal Ringer (curve 1) or low-Cl− Ringer solution in which 135 mM NaCl was replaced with 135 mM Na-glutamate (curve 2). (B) Whole-cell current responses to ramp pulses from −70 to +70 mV before and after replacement of extracellular Cl− with GSH. The pipette solution was Cl−-rich (125 mM CsCl); the bath solution was the normal Ringer (curve 1) or low-Cl− Ringer solution in which 135 mM NaCl was replaced with 135 mM Na-GSH (curve 2). (C) Instantaneous whole-cell I–V relationships in response to step pulses in 20 mV increments. The pipette solution was Cl−-rich (curve 1: 125 mM CsCl) or GSH−-rich (curve 2: 100 mM Cs-GSH plus 25 mM CsCl). The bath solution was the normal Ringer solution. Averaged data of 6 experiments performed in each configuration are plotted.