Figure 1.
Generation of an mVps34 gene (Pik3c3)-floxed mouse and deletion of mVps34 selectively in podocytes. (A) Strategy for making a conditional mVps34 knockout mouse. (B) Generation of renal glomerular podocyte-specific mVps34 knockout (mVps34pdKO) mice. (C) Podocin-Cre–mediated homologous recombination of mVps34 gene verified by PCR using isolated glomerular genomic DNA as template. (D) Effective deletion of mVps34 protein by podocin-Cre recombinase confirmed by immunoblotting analysis of isolated glomerular homogenates. FLPe, enhanced FLP; WT, wild type.