Figure 4.

aPKC double mutant mice display severely disturbed glomerular maturation. (A) Periodic acid–Schiff staining of P4 kidney sections of mice of the genotypes as indicated. Arrows mark mesangiolysis and mesangial hemorrhagia in aPKC double mutant mice. Asterisks mark tubular dilations with protein casts. (B) Ultrastructural analysis of P4 kidney sections reveals a regular foot process network in control and aPKCζ KO mice. In aPKCλ/ι PcKO mice, broadened foot processes with narrowed filtration slits (arrows) can be observed. In aPKC double mutant mice, podocytes (arrowheads) exhibit an undifferentiated appearance, sitting directly on the glomerular basement membrane. Asterisks indicate dilated capillaries, dotted line indicates the glomerular basement membrane, and white arrowhead indicates mesangiolysis. Scale bars, 20 µm in A; 2 µm in B.