Figure 5.

aPKC double mutant mice develop capillary aneurysms during glomerular maturation. (A) Frozen kidney sections of mice of the indicated genotypes at P4 are stained using antibodies against the mesangial marker desmin and the podocyte marker podocin and are subjected to confocal laser microscopy. Arrows indicate podocyte extensions to the mesangium, which regress during glomerular maturation in aPKC double mutant mice. (B) Frozen kidney sections of mice at P4 were stained using antibodies against the endothelial marker CD31 and the podocyte marker podocin. Arrows mark CD31-positive stained capillaries. In aPKC double mutant mice, massive aneurysms can be observed (asterisks). Scale bars, 5 µm.