Figure 6.

Impaired expression of slit diaphragm proteins in aPKC double mutant mice. (A–C) Staining of frozen kidney sections at P4 for the slit diaphragm proteins Par3, nephrin, podocin, and the basement membrane marker nidogen. Arrows mark expression of the indicated proteins, asterisks mark aneurysms, and arrowheads label mesangiolysis. (D) Staining of frozen kidney sections at P4 for the podocyte primary process marker detyrosinated tubulin and the slit diaphragm protein nephrin. Arrows mark expression of detyrosinated tubulin. Scale bars, 5 µm.