Figure 2.

Generation and characterization of the MHC-ANF-RFP transgenic reporter as a nephrocyte functional readout. (A–A″) The control MHC-RFP reporter is expressed in the muscles, reflecting the pattern of the MHC enhancer. (B–B″) The MHC-ANF-RFP reporter can only be observed in nephrocytes. (C–C″) The MHC-ANF-RFP reporter can only be observed in pericardial nephrocytes in adult flies. (D–F) The MHC-ANF-RFP reporter can also be found in garland nephrocytes (GNs) in (E and E′) late embryonic stage and (E and E′) early and (F and F′) late larval stages. Hand-GFP (green) was used to label both pericardial and garland nephrocytes.